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Gene Model: Adhfe1

NomenclatureGenomic Location
SymbolAdhfe1Chromosome1
NameAlcohol dehydrogenase
iron-containing protein 1		Linkage mapunknown
SpeciesDracomimus familiarisGenome CoordinatesChr1: 100 Mbp

Molecular Function

Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG.

Molecular Function Terms:

binding
   ion binding
      cation binding
         metal ion binding

catalytic activity
   oxidoreductase activity
      oxidoreductase activity, acting on CH-OH group of donors

Human Disease Association

No disease association.

Predicted Transcript
     1 GCCATGGCAGGGGGACGGGAGAGGGTCGCTCATTTGTTGAAGCAACTGCACCGAGCCTCA
    61 TGTGCGTGCCCTGCTCATTCCCATGTTTATTCACAAGGTATTGGACATGCTGCATCAGGG
   121 AAAACAGACTATGCTTTTGAGATGGCTGTTTCCAATATTCGATATGGAAAAGGAGTTACT
   181 AAAGAAGTGGGCATGGATCTGCATAATTTGGGTGCCAGAAAGGTTTGCGTGATGACAGAT
   241 AAAAACCTTTCTCAGCTCCCTCCTGTGAAAGCAGTTCTGGATTCTCTGACCACAAATGGT
   301 ATAAACTTCCAAATGTATGACAATGTCAGAGTGGAGCCAACAGACCAGAGCTTCATGGAT
   361 GCCATCATGTTCGCTAAAAAGGAAGAGTTTGATTCCTATGTCGCTGTTGGTGGAGGTTCG
   421 GTCATAGATACCTGTAAAACTGCCAATCTCTATGCATCCAGTCCAGAATCAGATTTTTTG
   481 GATTATGTTAATGCTCCAATTGGAAAAGGCAAGCCTGTGAACATCACTCTCAAACCATTG
   541 ATTGCAGTTCCTACAACTGCTGGAACTGGAAGTGAAACTACTGGAGTTGCTATTTTTGAT
   601 TATAAAGAACTAAAAGTAAAAACTGGCATTGCTTCCAGAACTATTAAGCCCTTATTGGGT
   661 ATAATTGACCCTCTACATACTCTTTATATGCCTGAGCGTGTGACAGCTAATACTGGCTTT
   721 GATGTGCTTTGTCATGCCCTCGAATCCTATACTGCCCTTCCTTACCATCAGCGTAGTCCC
   781 TGTCCTTCAAATCCAGTGAACCGACCAGCTTATCAAGGAAGTAATCCTATCAGTGATGTT
   841 TGGGCCATCCATGCATTGCGGATCACTGCTAAATATTTAAAGAGAGCCATCAGGAACCCC
   901 GAAGATGAAGAAGCAAGGGCTAGCATGCATCTAGCAAGTGCGTTTGCTGGCATTGGTTTT
   961 GGAAATGCTGGTGTGCATCTGTGTCATGGCATGTCTTACCCTATTTCCGGCTTGGTGAAG
  1021 AATTATAAAGCAAAGGATTATCGTGTGGAGCATTCTTTAGTGCCACATGGCCTTTCCGTT
  1081 GTCCTTACATCCCCAGCCGTGTTTACTTTCACAGCACCAATGTGTCCTGAGCGTCATTTG
  1141 GAAGCTGCCCATATACTGGGAGCTAACATCAGCAATTCCAAAGTCAGAGATGCTGGGCTT
  1201 ATTTTGGCAGACACCCTCCGGGAATTCTTGTTTGACCTGAATGTTGATGATGGCTTAGCT
  1261 GCTCTTGGTTATTCTTCAGCTGATATCCCTGCCTTAGTTAAAGGCACATTGCCCCAGGAA
  1321 CGAGTGACTAAATTATCTCCACGTCCCCAAACTGAAGAAGAATTATCTGCTCTGTTTGAA
  1381 GCTTCCATGAAACTGTATTAG

Predicted Protein Product
AMAGGRERVAHLLKQLHRASCACPAHSHVYSQGIGHAASGKTDYAFEMAVSNIRYGKGVT
KEVGMDLHNLGARKVCVMTDKNLSQLPPVKAVLDSLTTNGINFQMYDNVRVEPTDQSFMD
AIMFAKKEEFDSYVAVGGGSVIDTCKTANLYASSPESDFLDYVNAPIGKGKPVNITLKPL
IAVPTTAGTGSETTGVAIFDYKELKVKTGIASRTIKPLLGIIDPLHTLYMPERVTANTGF
DVLCHALESYTALPYHQRSPCPSNPVNRPAYQGSNPISDVWAIHALRITAKYLKRAIRNP
EDEEARASMHLASAFAGIGFGNAGVHLCHGMSYPISGLVKNYKAKDYRVEHSLVPHGLSV
VLTSPAVFTFTAPMCPERHLEAAHILGANISNSKVRDAGLILADTLREFLFDLNVDDGLA
ALGYSSADIPALVKGTLPQERVTKLSPRPQTEEELSALFEASMKLY
Protein Alignment to Mouse
sp|Q8R0N6|HOT_MOUSE Hydroxyacid-oxoacid transhydrogenase, mitochondrial OS=Mus
            musculus GN=Adhfe1 PE=2 SV=2
      MGI:1923437 Adhfe1 alcohol dehydrogenase, iron containing, 1 (Chr 1)
        Length = 465

 Score = 1944 (689.4 bits), Expect = 1.5e-201, P = 1.5e-201
 Identities = 368/465 (79%), Positives = 410/465 (88%)

Query:     3 AGGRERVAHLLKQLHRASCACPAHSHVYSQGIGHAASGKT-DYAFEMAVSNIRYGKGVTK 61
             A  R RV HLL+ L   +C CP HSH YSQ  G   SGKT DYAFEMAVSNIRYG GVTK
Sbjct:     3 AAARARVTHLLRHLQSTACQCPTHSHTYSQAPG--PSGKTADYAFEMAVSNIRYGAGVTK 60

Query:    62 EVGMDLHNLGARKVCVMTDKNLSQLPPVKAVLDSLTTNGINFQMYDNVRVEPTDQSFMDA 121
             EVGMDL N+GA+ VC+MTDKNLSQLPPV+ V+DSL+ NGI+FQ+YD+VRVEPTD SFMDA
Sbjct:    61 EVGMDLQNMGAKNVCLMTDKNLSQLPPVQIVMDSLSKNGISFQVYDDVRVEPTDGSFMDA 120

Query:   122 IMFAKKEEFDSYVAVGGGSVIDTCKTANLYASSPESDFLDYVNAPIGKGKPVNITLKPLI 181
             I FAKK  FD+YVAVGGGS +DTCK ANLYASSP S+FLDYVNAPIGKGKPV + LKPLI
Sbjct:   121 IEFAKKGAFDAYVAVGGGSTMDTCKAANLYASSPHSEFLDYVNAPIGKGKPVTVPLKPLI 180

Query:   182 AVPTTAGTGSETTGVAIFDYKELKVKTGIASRTIKPLLGIIDPLHTLYMPERVTANTGFD 241
             AVPTT+GTGSETTGVAIFDY+ LKVKTGIASR IKP LG++DPLHTL+MP +V AN+GFD
Sbjct:   181 AVPTTSGTGSETTGVAIFDYEHLKVKTGIASRAIKPTLGLVDPLHTLHMPCQVVANSGFD 240

Query:   242 VLCHALESYTALPYHQRSPCPSNPVNRPAYQGSNPISDVWAIHALRITAKYLKRAIRNPE 301
             VLCHALESYTA+PY  RSPCPSNP+ RPAYQGSNPISD+WA+HAL+I AKYLKRA+RNP+
Sbjct:   241 VLCHALESYTAIPYSMRSPCPSNPIQRPAYQGSNPISDIWAVHALQIVAKYLKRAVRNPD 300

Query:   302 DEEARASMHLASAFAGIGFGNAGVHLCHGMSYPISGLVKNYKAKDYRVEHSLVPHGLSVV 361
             D EAR+ MHLASAFAGIGFGNAGVHLCHGMSYPISGLVK YKAK+Y V+H LVPHGLSVV
Sbjct:   301 DLEARSKMHLASAFAGIGFGNAGVHLCHGMSYPISGLVKTYKAKEYNVDHPLVPHGLSVV 360

Query:   362 LTSPAVFTFTAPMCPERHLEAAHILGANISNSKVRDAGLILADTLREFLFDLNVDDGLAA 421
             LTSPAVFTFTA M PERHLE A ILGANI  ++++DAGL+LAD LR+FLFDLNVDDGLAA
Sbjct:   361 LTSPAVFTFTAQMFPERHLETAGILGANIRTARIQDAGLVLADALRKFLFDLNVDDGLAA 420

Query:   422 LGYSSADIPALVKGTLPQERVTKLSPRPQTEEELSALFEASMKLY 466
             LGYS  DIP+LVKGTLPQERVTKL+PR Q+EE+LSALFEASMKLY
Sbjct:   421 LGYSKDDIPSLVKGTLPQERVTKLAPRAQSEEDLSALFEASMKLY 465